Lack o f Light Regulation of N A D P H : Protochlorophyllide Oxido - Reductase m RNA in Cress Seedlings ( Lepidium sativum L . )
نویسندگان
چکیده
A cDNA clone for NADPH : protochlorophyllide oxi doreductase from barley was subcloned for production of antisense-mRNA. This enabled heterologous hybridi zation with RNA from cress seedlings (Lepidium sativum L.). The mRNA level for N A DPH : protochlorophyllide oxidoreductase did not decrease in cress seedlings during irradiation with continuous far-red or white light up to 12 h. The amount of NADPH : protochlorophyllide oxi doreductase protein, identified by Western blot de creased 5-fold after continuous irradiation with white light for 12 h. Species differences for light regulation of RNA are discussed.
منابع مشابه
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